Department Equipment

Protein Expression & Purification

• Incubators

  • Bellco, Insect Cell Incubator
    • Barnstead, Stir Plates (7)
    • Corning, Spinner Flasks
  • Thermo, Max Q5000 Floor Shaking Incubator (Bacteria))
  • Thermo, Max Q5000 Floor Shaking Incubator (Yeast)

• Fermentor & Bioreactor

  • Eppendorf, BioFlo/Celligen-115

• Sonicator

  • Branson, Sonifer

• High Speed Centrifuge

  • Beckman, Avanti JXN-26 Centrifuge
    • Beckman, Avanti J26XPI Centrifuge
    • Beckman, JLA-8.1000 Rotor

• FPLCs

  • GE, AKTA Explorer A100 FPLC with MALS and RI detector
    • Wyatt, Dynamic Light Scattering (DynoPro NanoStar)
    • Wyatt, Multi-angle Light Scattering (miniDAWN TREOS)
    • Wyatt, Optilab rEX (RI detector)
  • Shimadzu, CBM-20A HPLC

• Spectrophotometers

  • Beckman, DU-640 Spectrophotometer
  • Beckman, DU-730 UV-Vis Spectrophotometer

• Water Purifcation System

  • Siemens, Modulab Water Purification System

• Biological Safety Cabinet

  • Thermo-Fisher, Biological Safety Cabinet (1300 Series Class II, Type A2)

• Filtration and Concentration

  • Tangential Flow Filtration

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Protein Biophysics

• Protein Characterization

  • AVIV, Circular Dichroism Spectrometer
  • Biacore, T200
  • Microcal, Isothermal Titration Calorimeter (ITC200)
  • Monolith NT.115 Microscale Thermophoresis (Nano Temper Technologies)
  • Tecan Spark
    • Tecan, Infinite M1000 Quadruple Monochromator Microplate Reader
  • Zetasizer Nano-ZS

• Spectrometer

  • Bruker, EPR Spectrometer

• Spectrophotometer

  • Cary, 100 Bio UV Visible Spectrophotometer

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Automated Crystallization

• Liquid Handlers

  • HydraDT - Liquid Handler
  • Mosquito - Liquid Handler/Spotter
  • Tecan, Freedom Evo - Liquid Handler

• Protein Crystal Chambers

  • Formulatrix, Rock Imager 500
  • Powers, Protein Crystallography Incubators (2)

• Plate Sealer

  • Thermo, ALPS 50V - Manual Heat sealer

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Imaging

• Microscopes

  • Leica, M205A Stereomicroscope
  • Leica, S8 APO
  • Olympus, BX51 Upright Epifluorescence Microscope

• Film Processor

  • Konica Minolta SRX-101A Processor

• Image Aquisition & Documentation Systems

  • GE, Typhoon Imager
  • ProteinSimple, FluorChem E

• CellTirf Microscope

  • Olympus, CellTIRF Microscope

• Confocal Microscope

  • Olympus, FluoView Confocal FV1000

• Ultrasound Imaging System

  • Fujifilm, Vevo 3100

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Stopped Flow

• Stopped Flow Spectrometer

  • Applied Photophysics, SX20 Stopped Flow Spectrometer

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Centrifugation

• High Speed Centrifuges

  • Beckman, Avanti J-E Centrifuge [2 - E613]
  • Beckman, Avanti J-E Centrifuge [4 - E537]

• High Speed Centrifuge Rotors

  • Beckman, JA-10 Fixed Angle Rotor
  • Beckman, JA-12 Fixed Angle Rotor
  • Beckman, JA-20 Fixed Angle Rotors (5)
  • Beckman, JA-25-50 Fixed Angle Rotor
  • Beckman, JS-5.3 Swining Bucket Rotors (3)
  • FIBERLite, F14BCI-6x250y Rotors (2)
  • FIBERLite, F10BCI-6x500y Rotor

• Ultracentrifuges

  • Beckman, Optima L-100XP Preperative Ultracentrifuge
  • Beckman, Optima XPN-100 Preparative Ultracentrifuge

• Ultracentrifuge Rotors

  • Beckman, NVT-65 Rotor (E537)
  • Beckman, SW 32 Ti Rotor (E537)
  • Beckman, Type 45 Ti Rotors, E537 & E613
  • Beckman, Type 50.4 Ti Rotor, E537 & E613
  • Beckman, Type 70 Ti Rotor, E537 & E613
  • Beckman, Type 90 Ti Rotor, E537 & E613

• Tabletop Ultracentrifuge

  • Beckman, Optima MAX-XP Tabletop Ultracentrifuge
    • Beckman, TLA 100.3 Rotor
    • Beckman, TLA 120.1 Rotor

• Benchtop Centrifuges

  • Beckman, Allegra 25R Centrifuge_2
  • Beckman, Allegra X-15R

• Benchtop Centrifuge Rotors

  • Beckman, TA-14-50 Rotor
  • Beckman, TA-15-1.5 Rotor
  • Beckman, TS-5.1-500 Rotor, Swinging Bucket Rotor

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Cell Culture - North

• Incubators

  • Forma, 3110 CO2 Incubator
  • Forma, 3158 CO2 Incubator
  • Forma, 3326 CO2 Incubator
  • Thermo/NAPCO, 3589 CO2 Incubator
  • Thermo/NAPCO, 3589 CO2 Incubator

• Microscopes

  • Leitz, DM-IL Microscope
  • Nikon, Eclipse TS100 Microscope

• Benchtop Centrifuge

  • Eppendorf, Cell Culture Centrifuge

• Biological Safety Cabinets

  • Forma Scientific, 1202 Class II Type A1 Biosafety Cabinet
  • Forma Scientific, 1284 Class II Type A/B3 Biosafety Cabinet
  • Thermo Scientific, 1300 Class II, A2 Biosafety Cabinet

• Cell Culture Hypoxia Incubator Chamber

  • Coy, Hypoxia Chamber for Cell Culture

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Cell Culture BSL-2 - North

• Incubator

  • Forma, Steri-Cycle CO2 Incubator

• Microscope

  • Olympus, CH-2 Microscope

• Biological Safety Cabinet

  • Labconco Class II Type A2 Biosafety Cabinet

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Cell Culture - South

• Biosafety Clean Bench

  • Forma Scientific, Clean Bench

• Incubators

  • NAPCO, CO2 Incubators
  • Thermo Fisher 4110 CO2 Incubator
  • Thermo Fisher 4140 Incubator
  • Thermo Forma, 3140 CO2 - O2 Incubator
  • Thermo Forma, 3140 CO2 - O2 Incubator

• Microscope

  • Zeiss, Axiovert 25
    • Zeiss, Q-Imaging QICAM

• Benchtop Centrifuge

  • Beckman, Allegra 25R Centrifuge

• Biological Safety Cabinets

  • Baker, BioGard Hood
  • LabConco Biosafety Hood
  • NuAir, Bisafety Hood

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Oocyte Cell Culture & Expression - South

• Biosafety Clean Bench

  • Labconco, Biosafety Clean Bench

• Incubator

  • Torrey Pines, IN40 Oocyte Incubator

• Oocyte injection System

  • Drummond, Nanoject II - Oocyte injection Stations (3)

• Automated 2 Electrode Voltage Clamp

  • Robocyte 2 - Automated 2EVC system

• Automated Oocyte Injection

  • Roboinject - Automated Oocyte Injection

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Microplate Spectrophotometry

• Luminometer

  • Luminoskan Ascent

• Microplate Reader

  • SpectraMAX 340

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PCR

• Real-Time PCR Thermocycler

  • ABI, StepOnePlus Real-Time PCR System

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Mouse Physiology & Phenotyping

• Exercise Wheels

  • Lafayette, Exercise Wheels

• Hematology Analyzer

  • Heska, HemaTrue

• Environmental Chambers

  • Biospherix, Oxycycler

• Blood Chemistry Analyzer

  • Heska, DRI-CHEM 7000

• Blood Gas Analyzer

  • Nova, STAT PROFILE Prime

• Telemetry System

  • DSI, Telemetry Unit

• Blood Pressure System

  • Kent, Blood Pressure System

• Metabolic Cage

  • Columbus, Metabolic Cage System

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General Equpimment

• Gas Manifolds

  • Beacon-Medaes, Carbon Dioxide Manfold
  • Beacon-Medaes, Nitrogen Manifold

• Water Purifcation System

  • Milli-Q Water system (7)

• Dish Washers

  • Labconco, FlaskScrubber washer
  • Labconco, SteamScrubber

• Autoclaves

  • Amsco, Century Steam Sterilizer
  • Amsco, Eagle Series Autoclave
  • Amsco, Lab 250 Autoclave

• Ice Maker

  • Hoshizaki, Ice Machine

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